Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized calu-3 lung epithelial cells
Identifieur interne : 004E71 ( Main/Exploration ); précédent : 004E70; suivant : 004E72Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized calu-3 lung epithelial cells
Auteurs : Chien-Te K. Tseng [États-Unis] ; Jennifer Tseng [États-Unis] ; Lucy Perrone [États-Unis] ; Melissa Worthy [États-Unis] ; Vsevolod Popov [États-Unis] ; Clarence J. Peters [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- Antigènes viraux (métabolisme), Cellules épithéliales (physiologie), Cellules épithéliales (virologie), Effet cytopathogène viral, Humains, Lignée cellulaire tumorale, Polarité de la cellule (physiologie), Poumon, Récepteurs viraux (métabolisme), Syndrome respiratoire aigu sévère (virologie), Technique d'immunofluorescence, Virus du SRAS (croissance et développement), Virus du SRAS (métabolisme).
- MESH :
- croissance et développement : Virus du SRAS.
- métabolisme : Antigènes viraux, Récepteurs viraux, Virus du SRAS.
- physiologie : Cellules épithéliales, Polarité de la cellule.
- virologie : Cellules épithéliales, Syndrome respiratoire aigu sévère.
- Pascal (Inist)
English descriptors
- KwdEn :
- Antigens, Viral (metabolism), Cell Line, Tumor, Cell Polarity (physiology), Coronavirus, Cytopathogenic Effect, Viral, Epithelial Cells (physiology), Epithelial Cells (virology), Epithelial cell, Fluorescent Antibody Technique, Humans, Lung, Microbiology, Receptors, Virus (metabolism), Release, SARS Virus (growth & development), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Virology.
- MESH :
- chemical , metabolism : Antigens, Viral, Receptors, Virus.
- growth & development : SARS Virus.
- metabolism : SARS Virus.
- physiology : Cell Polarity, Epithelial Cells.
- virology : Epithelial Cells, Severe Acute Respiratory Syndrome.
- Cell Line, Tumor, Cytopathogenic Effect, Viral, Fluorescent Antibody Technique, Humans, Lung.
Abstract
Severe acute respiratory syndrome (SARS), caused by a novel coronavirus (CoV) known as SARS-CoV, is a contagious and life-threatening respiratory illness with pneumocytes as its main target. A full understanding of how SARS-CoV would interact with lung epithelial cells will be vital for advancing our knowledge of SARS pathogenesis. However, an in vitro model of SARS-CoV infection using relevant lung epithelial cells is not yet available, making it difficult to dissect the pathogenesis of SARS-CoV in the lungs. Here, we report that SARS-CoV can productively infect human bronchial epithelial Calu-3 cells, causing cytopathic effects, a process reflective of its natural course of infection in the lungs. Indirect immunofluorescence studies revealed a preferential expression of angiotensin-converting enzyme 2 (ACE-2), the functional receptor of SARS-CoV, on the apical surface. Importantly, both ACE-2 and viral antigen appeared to preferentially colocalize at the apical domain of infected cells. In highly polarized Calu-3 cells grown on the membrane inserts, we found that cells exposed to virus through the apical rather than the basolateral surface showed high levels of viral replication. Progeny virus was released into the apical chamber at titers up to 5 logs higher than those recovered from the basolateral chambers of polarized cultures. Taken together, these results indicate that SARS-CoV almost exclusively entered and was released from the apical domain of polarized Calu-3 cells, which might provide important insight into the mechanism of transmission and pathogenesis of SARS-CoV.
Affiliations:
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Peters</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, University of Texas Medical Branch</s1><s2>Galveston, Texas 77555-0609</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Galveston, Texas 77555-0609</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Pathology, University of Texas Medical Branch</s1><s2>Galveston, Texas 77555-0609</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Galveston, Texas 77555-0609</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, Viral (metabolism)</term><term>Cell Line, Tumor</term><term>Cell Polarity (physiology)</term><term>Coronavirus</term><term>Cytopathogenic Effect, Viral</term><term>Epithelial Cells (physiology)</term><term>Epithelial Cells (virology)</term><term>Epithelial cell</term><term>Fluorescent Antibody Technique</term><term>Humans</term><term>Lung</term><term>Microbiology</term><term>Receptors, Virus (metabolism)</term><term>Release</term><term>SARS Virus (growth & development)</term><term>SARS Virus (metabolism)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Severe acute respiratory syndrome</term><term>Virology</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antigènes viraux (métabolisme)</term><term>Cellules épithéliales (physiologie)</term><term>Cellules épithéliales (virologie)</term><term>Effet cytopathogène viral</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Polarité de la cellule (physiologie)</term><term>Poumon</term><term>Récepteurs viraux (métabolisme)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Technique d'immunofluorescence</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Viral</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes viraux</term><term>Récepteurs viraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cellules épithéliales</term><term>Polarité de la cellule</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Polarity</term><term>Epithelial Cells</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term><term>Cytopathogenic Effect, Viral</term><term>Fluorescent Antibody Technique</term><term>Humans</term><term>Lung</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Effet cytopathogène viral</term><term>Humains</term><term>Libération</term><term>Cellule épithéliale</term><term>Lignée cellulaire tumorale</term><term>Microbiologie</term><term>Poumon</term><term>Technique d'immunofluorescence</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS), caused by a novel coronavirus (CoV) known as SARS-CoV, is a contagious and life-threatening respiratory illness with pneumocytes as its main target. A full understanding of how SARS-CoV would interact with lung epithelial cells will be vital for advancing our knowledge of SARS pathogenesis. However, an in vitro model of SARS-CoV infection using relevant lung epithelial cells is not yet available, making it difficult to dissect the pathogenesis of SARS-CoV in the lungs. Here, we report that SARS-CoV can productively infect human bronchial epithelial Calu-3 cells, causing cytopathic effects, a process reflective of its natural course of infection in the lungs. Indirect immunofluorescence studies revealed a preferential expression of angiotensin-converting enzyme 2 (ACE-2), the functional receptor of SARS-CoV, on the apical surface. Importantly, both ACE-2 and viral antigen appeared to preferentially colocalize at the apical domain of infected cells. In highly polarized Calu-3 cells grown on the membrane inserts, we found that cells exposed to virus through the apical rather than the basolateral surface showed high levels of viral replication. Progeny virus was released into the apical chamber at titers up to 5 logs higher than those recovered from the basolateral chambers of polarized cultures. Taken together, these results indicate that SARS-CoV almost exclusively entered and was released from the apical domain of polarized Calu-3 cells, which might provide important insight into the mechanism of transmission and pathogenesis of SARS-CoV.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Tseng, Chien Te K" sort="Tseng, Chien Te K" uniqKey="Tseng C" first="Chien-Te K." last="Tseng">Chien-Te K. Tseng</name></noRegion><name sortKey="Perrone, Lucy" sort="Perrone, Lucy" uniqKey="Perrone L" first="Lucy" last="Perrone">Lucy Perrone</name><name sortKey="Peters, Clarence J" sort="Peters, Clarence J" uniqKey="Peters C" first="Clarence J." last="Peters">Clarence J. Peters</name><name sortKey="Peters, Clarence J" sort="Peters, Clarence J" uniqKey="Peters C" first="Clarence J." last="Peters">Clarence J. Peters</name><name sortKey="Popov, Vsevolod" sort="Popov, Vsevolod" uniqKey="Popov V" first="Vsevolod" last="Popov">Vsevolod Popov</name><name sortKey="Tseng, Chien Te K" sort="Tseng, Chien Te K" uniqKey="Tseng C" first="Chien-Te K." last="Tseng">Chien-Te K. Tseng</name><name sortKey="Tseng, Jennifer" sort="Tseng, Jennifer" uniqKey="Tseng J" first="Jennifer" last="Tseng">Jennifer Tseng</name><name sortKey="Worthy, Melissa" sort="Worthy, Melissa" uniqKey="Worthy M" first="Melissa" last="Worthy">Melissa Worthy</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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